Professional Summary
- 10 years of experience in molecular biology, biochemistry, and assay development
- Expression of monoclonal/ bispecific antibodies & soluble proteins in suspension CHO/ HEK cells
- Development of high-producing stable clonal cell lines for protein production
- Experience with fed-batch for antibody and protein production
- Experience with developing and optimizing scale up process steps
- Knowledge of primary recovery (harvest/clarification) and purification of biologics
- Experience with library generation & screening (yeast and phage) and affinity maturation
- Develop and optimize AKTA purification protocols for antibodies and soluble proteins
- Characterize purity and homogeneity of the purified proteins using SEC and Bioanalyzer
- Experience with running developability assays (CIC, PSR, CSI-BLI,, HIC, DSF, BVP)
- Characterize antibodies using SPR (Biacore) and BLI (OCTET)
- Experience with flow cytometry (8 colors)
- Proficient in Data analysis, statistical tests, visualization, and presentation (R/ RStudio)
- Effective communication, organizational skills, documentation
Senior Research Scientist, MassBiologics
2020 - Present
- Project lead in developing antibodies and proteins for non-human primate (NHP) preclinical studies.
- Design, production, purification, and QC of monoclonal, bispecific antibodies, & soluble proteins
- Generation of stable, high titer clonal suspension CHO & HEK cell lines
- Running transient and fed-batches to produce and purify antibodies & soluble proteins
- Developing and optimizing scale up process steps (upto 4L)
- Development, screening and validation of yeast and phage VHH & scFv libraries
- Multicolor flow cytometry (8 color) and panel generation in primate blood/ PBMCs
- Characterization of antibody kinetics using SPR and BLI.
- Running developability assays (CIC, PSR, CSI-BLI,, HIC, DSF, BVP)
- Establishing new and reviewing SOPs and laboratory protocols
- Training colleagues & research interns; weekly progress reports
Scientist II, Totient Inc.
2018 - 2020
- Transient and fed-batch production of antibodies in suspension CHO cell lines
- Production and characterization of scFvs in bacterial cells
- Identification of targets for antibodies and scFvs using ELISA and SPR
- Establishing new and reviewing SOPs and laboratory protocols
Postdoctoral Associate, University of Massachusetts Medical School
2018
- CRISPR screens to identify novel epigenetic regulators in Ewing’s Sarcoma
- Execution of genome wide shRNA/ RNAi and proteomic screens
- Genome editing by CRISPR/Cas9 for specific projects in the lab