Shubham Dutta
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Experience

Professional Summary

  • 10 years of experience in molecular biology, biochemistry, and assay development
  • Expression of monoclonal/ bispecific antibodies & soluble proteins in suspension CHO/ HEK cells
  • Development of high-producing stable clonal cell lines for protein production
  • Experience with fed-batch for antibody and protein production
  • Experience with developing and optimizing scale up process steps
  • Knowledge of primary recovery (harvest/clarification) and purification of biologics
  • Experience with library generation & screening (yeast and phage) and affinity maturation
  • Develop and optimize AKTA purification protocols for antibodies and soluble proteins
  • Characterize purity and homogeneity of the purified proteins using SEC and Bioanalyzer
  • Experience with running developability assays (CIC, PSR, CSI-BLI,, HIC, DSF, BVP)
  • Characterize antibodies using SPR (Biacore) and BLI (OCTET)
  • Experience with flow cytometry (8 colors)
  • Proficient in Data analysis, statistical tests, visualization, and presentation (R/ RStudio)
  • Effective communication, organizational skills, documentation

Senior Research Scientist, MassBiologics

2020 - Present
  • Project lead in developing antibodies and proteins for non-human primate (NHP) preclinical studies.
  • Design, production, purification, and QC of monoclonal, bispecific antibodies, & soluble proteins
  • Generation of stable, high titer clonal suspension CHO & HEK cell lines
  • Running transient and fed-batches to produce and purify antibodies & soluble proteins
  • Developing and optimizing scale up process steps (upto 4L)
  • Development, screening and validation of yeast and phage VHH & scFv libraries
  • Multicolor flow cytometry (8 color) and panel generation in primate blood/ PBMCs
  • Characterization of antibody kinetics using SPR and BLI.
  • Running developability assays (CIC, PSR, CSI-BLI,, HIC, DSF, BVP)
  • Establishing new and reviewing SOPs and laboratory protocols
  • Training colleagues & research interns; weekly progress reports

Scientist II, Totient Inc.

2018 - 2020
  • Transient and fed-batch production of antibodies in suspension CHO cell lines
  • Production and characterization of scFvs in bacterial cells
  • Identification of targets for antibodies and scFvs using ELISA and SPR
  • Establishing new and reviewing SOPs and laboratory protocols

Postdoctoral Associate, University of Massachusetts Medical School

2018
  • CRISPR screens to identify novel epigenetic regulators in Ewing’s Sarcoma
  • Execution of genome wide shRNA/ RNAi and proteomic screens
  • Genome editing by CRISPR/Cas9 for specific projects in the lab