Professional Summary
- 10+ years of experience in experiment design and assay development
- Experience antibody and soluble protein developability, production, purification & QC
- Experience in CRISPR-based gene editing in mammalian cell lines (KO and KI)
- Development and selection of stable clonal cell lines using PiggyBac system
- Experience in hybridoma, yeast display screen, and 8-color flow cytometry
- AKTA based purification and optimization of proteins using affinity columns
- Experience with antibody target validation and affinity maturation
- Characterize protein interaction and kinetics using ELISA, SPR, and BLI
- Proficient in data analysis, statistical tests, visualization, and presentation in R/ Python
- Establishing new and reviewing SOPs and laboratory protocols
- Effective communication, organizational skills, record keeping
- Ability to learn quickly, work independently, and train colleagues
Senior Research Scientist, MassBiologics
2020 - Present
- Project manager and lead in developing novel antibodies and proteins for non-human primate (NHP) preclinical studies
- Optimization of bispecific, multispecific, and alternative antibody formats
- Multicolor flow cytometry and panel generation for primate blood
- Generation of high producing stable clonal cell lines for antibodies and protein production
- Improve antibody yield and function by gene editing
- Characterizing protein-protein interaction using ELISA and kinetics using SPR and BLI
- Establishing new and reviewing SOPs and laboratory protocols
- Training colleagues & research associates
Scientist II, Totient Inc.
2018 - 2020
- Served as cell and molecular biology lead for oncology drug discovery programs
- Developed strategies to simultaneously knockout two genes using CRISPR-Cas9
- Experience with preparation of high-quality whole genome lentiviral sgRNA particles
- Identification of targets using CRISPR mediated whole genome knockout screen on multiple cell lines
- Identification of targets for recombinant antibodies using Protein arrays, ELISA and immunofluorescence
- Worked with CROs with for special assays including 3D cell culture and SPR (Biacore)
- Managed one direct report
Postdoctoral Associate, University of Massachusetts Medical School
2018
- Developing a CRISPR screen to identify novel epigenetic regulators in Ewing’s Sarcoma
- Executing genome wide shRNA/ RNAi and proteomic screens to identify regulators in X-chromosome inactivation pathway
- Genome editing by CRISPR/Cas9 for specific projects in the lab
- Managed one intern and two junior graduate students