Skills

Molecular Biology

Plasmid preparation (mini to giga), sequencing, cloning, expression, preparation of NGS libraries, transfection, PCR, ddPCR, qPCR, Western blot, confocal imaging, RNAi and CRISPR screens

Protein Biology

Antibody production using transient and Fed-Batch culture in suspension cells, Purification using AKTA, Harvest and clarification, ELISA, Process optimization and scale up, Yeast and phage library screening, Affinity maturation, Antibody developability assays (CIC, PSR, CSI-BLI, HIC, DSF, BVP), Hybridoma screening,Surface Plasmon resonance (SPR) using Biacore, BiLayer Interferometry (BLI) using OCTET, In vitro Protein purification and kinase assays, Immunoprecipitation

Cell line development

Stable cell line generation (CHO, HEK, MCF10A, etc.) using conventional and PiggyBac system, Electroporation, High-throughput clone selection (Clonepix, Duetz system), Handling and culture of blood and PBMCs, Viral packaging and transduction, Soft agar colony formation, invasion and migration assays, Mammalian Cell culture, maintenance, Transfection and related techniques

Data Science

Electronic notebook keeping using Benchling, R programming (Base, Tidyverse, Shiny, Bioconductor), Data analysis and visualization, Statistics using R, Data driven websites (using R Shiny, RMarkdown, Quarto), Basic knowledge in Python programming

Scientific Editing/ Writing

Scientific papers and research proposals for grants, Editorial Board member of several peer-reviewed journals